The Gadsden Flag, designed in 1775, showcases a coiled rattlesnake with the word "Don't Tread on Me

https://mylespgzl078.cavandoragh.org/old-glory-is-beautiful-a-love-letter-to-the-stars-and-stripes

The Gadsden Flag, designed in 1775, showcases a coiled rattlesnake with the word "Don't Tread on Me," embodying American resistance opposed to tyranny and oppression.